Molecular Biology Reagents and Kits
Sigma Aldrich Fine Chemicals Biosciences CRISPR LacZ Positive Contr1EA
Recent publications using CRISPR/Cas9-mediated recombineering in E. coli tout editing efficiencies near 100% making CRISPR/Cas9-mediated recombineering the most powerful bacterial genome engineering method to date. In addition Cas9-mediated recombineering overcomes the dependence on a second recombination step avoids the creation of destabilizing scar sites can be used in multiplexing and is less time-consuming than previous protocols.Here we present a novel dual-vector CRISPR/Cas-mediated»-Red system for improved recombineering in E. coli. Our system is shown to facilitate homology-directed repair of DSBs created by Cas9 endonuclease enabling genetic alterations through chromosomal integration of a donor DNA.This plasmid is to be used in combination with the Cas9 Lambda Red homologous recombination plasmid for E. coli (CAS9BAC1P) as the positive control for your custom gene editing experiment. The custom gRNA (CRISPRBACD) can be designed and ordered through https//www.sigmaald
Echelon Biosciences Research Labs PI(4,5)P2 PIP Beads 1mL
PI(4,5)P2 PIP BeadsTM are composed of agarose with 10 nanomoles of bound PI(4,5)P2 per mL of beads, enough for several protein binding experiments. Each 1 mL order of PIP Beads includes a small amount of control beads. Larger quantities of Control Beads are also available for purchase. This item is non returnable
Electron Microscopy Sciences Aurion ImmunoGold Reagents Custom Kit
Immunogold reagent kit. Two non-overlapping gold particle sizes, Coupled to: either Goat-anti-Rabbit and Goat-anti-Mouse IgG/IgM antibodies. Affinity purified and cross adsorbed antibodies.
Echelon Biosciences Research Labs Ins(1,5)P2 1 mg
Inositol phosphates are important signaling molecules involved in numerous cellular functions. Inositol 1,5-bisphosphate (Ins(1,5)P2) is not an intermediate in the inositol phosphate pathway but corresponds to the PtdIns(5)P headgroup. This item is non returnable
Echelon Biosciences Research Labs PI(3)P PolyPIPosomes 0.25 mL
PolyPIPosomes are polymerized liposomes that show markedly increased stability (up to 6 months) vs. conventional liposomes. A biotin tag is incorporated for easy detection of PIPn-binding proteins with streptavidin reagents. PolyPIPosomes are provided as a 1mM total lipid solution containing 5% PIP. Each order of 250 L or 1 mL of PolyPIPosomes includes 100 L of Control PolyPIPosomes. This item is non returnable
Medchemexpress LLC HN-saponin F | 39524-13-5 | 766.95 | 5 MG
HN-saponin F is an orally active triterpenoid saponin exhibiting anti-inflammatory activity. This compound is intended for research use only and has been tested to comply with specifications, showing a high purity of 98.45% (HPLC). It is stable under recommended storage conditions.
- Orally active triterpenoid saponin
- Anti-inflammatory activity
- For research use only
- High purity (98.45% by HPLC)
- Stable under recommended storage conditions
- Identified uses include laboratory chemicals and substance manufacture
Abcam 5-TRITC (Tetramethylrhodamine-5-isothiocyanate), Amine and nucleic acid reactive fluorescent probe, 25MG
MW 443.52 g/mol, Purity >95%. Amine and nucleic acid reactive fluorescent probe. 6-TRITC (ab145403) and 5(6)-TRITC (ab145353) S-isomer. Spectrally similar to TAMRA (ab145282, ab145438, ab145338, ab146486, ab145334, ab145433). Biodistributes covalently linked to poly(lactic-co-glycolic) acid (PLGA) and PLGA/chitosan nanoparticles after oral administration.
The product is subject to the following: Abcam Restricted Use Statement
![Zymo Research Corporation ZymoBIOMICS 96 DNA Kit (2 x 96 preps)Includes: [D4307] ZymoBIOMICS 96 DNA Kit x 1, [S6002-96-4] ZR BashingBead Lysis Racks (0.1mm & 0.5 mm) 2 pack x 1[D4300-1-150] ZymoBIOMICS Lysis Solution, 150 ml x 1](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00062073-D4303-ZYMOBIOMICS-96-DNA-KIT.JPG-250.jpg)
Zymo Research Corporation ZymoBIOMICS 96 DNA Kit (2 x 96 preps)Includes: [D4307] ZymoBIOMICS 96 DNA Kit x 1, [S6002-96-4] ZR BashingBead Lysis Racks (0.1mm & 0.5 mm) 2 pack x 1[D4300-1-150] ZymoBIOMICS Lysis Solution, 150 ml x 1
The ZymoBIOMICS™ innovative lysis system eliminates bias associated with unequal lysis efficiencies1 of different organisms (e.g. Gram-negative/positive bacteria including endospores2 , fungi, protozoans, algae, etc.) making it ideal for microbial community profiling. Unbiased mechanical lysis of tough microbes is achieved by bead beating with the innovative ultra-high density BashingBeads™ and validated using the ZymoBIOMICS™ Microbial Community Standard.In addition, the ZymoBIOMICS™ 96 DNA Kit is equipped with Zymo Researchs proprietary OneStep™ PCR InhibitorRemoval technology, enabling PCR from the most PCR prohibitive environmental samples rich in humic and fulvic acids, tannins, melanin, and other polyphenolic compounds. Coupling state-of-the-art lysis technology with Zymo-Spin™ Technology results in superior yields of ultra-pure DNA ideal for all downstream applications including PCR, arrays, 16S rRNA gene sequencing, and shotgun sequencing.